Acknowledgments We thank T. J Cell Biochem Antibodies for actin and histone H3 were used for the normalization of cytoplasmic and nuclear extracts, respectively. National Institutes of Health to Y. Address all correspondence and requests for reprints to: Received May 8; Accepted Sep Furthermore, we clearly demonstrate that NFATc1 induces expression of the key fusion-mediating molecules Atp6v0d2 and DC-STAMP, and that overexpression of these genes rescues the formation of multinuclear osteoclasts in CsA-treated cultures of mononuclear osteoclast precursors.
Nat Rev Genet 4: Closed boxes indicate putative NFATc1-binding sites. After 48 h of transfection, the cells were washed twice with PBS and then lysed in passive lysis buffer Promega.
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